Antibody Single Chain Optimize Expression

Input:

• List of sequences

• File name for output

Output:

• A csv file showing the mutations

• Positions, and expression fitness ranking - can be downloaded or viewed in the data tab

Description:

Runs antibody optimisation on an antibody variable region sequence and generates single substitution optimisation sequences. Multi sequence alignment (MSA) is run on a database of related germline sequences, then the results of this are sent to an LLM which produces a suggestion framework region. It then predicts the perplexity and fitness scores of these antibodies, and returns sequence and the perplexity in a csv file.